View source for Quantitation by concatenated tryptic peptides

{{Final
|acronym=([[QCAT]])
|def=Method for absolute quantitation of a protein or proteins in a multiplexed fashion using isotope-labeled peptides as [[surrogate internal standard]]s. The labeled peptides are produced by synthesizing a gene coding for all desired peptides, expressing this gene in ''E. coli'' culture containing appropriately labeled substrate, and digesting with trypsin the expressed protein composed of the desired concatenated peptides.
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|ref=R. J. Beynon, M. K. Doherty, J. M. Pratt, S. J. Gaskell. Nature Meth. 2, 587 (2005). (http://dx.doi.org/10.1038/nmeth774 ) 
}}
[[Category:Proteomics]]