Quantitation by concatenated tryptic peptides: Difference between revisions
From Mass Spec Terms
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Revision as of 11:19, 8 January 2014
| IUPAC RECOMMENDATIONS 2013 |
| Quantitation by concatenated tryptic peptides (QCAT) |
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| Method for absolute quantitation of a protein or proteins in a multiplexed fashion using isotope-labeled peptides as surrogate internal standards. The labeled peptides are produced by synthesizing a gene coding for all desired peptides, expressing this gene in E. coli culture containing appropriately labeled substrate, and digesting with trypsin the expressed protein composed of the desired concatenated peptides. |
| Related Term(s): |
| Reference(s):
R. J. Beynon, M. K. Doherty, J. M. Pratt, S. J. Gaskell. Nature Meth. 2, 587 (2005). (http://dx.doi.org/10.1038/nmeth774 ) |
| From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013. |
