Shotgun proteomics: Difference between revisions
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|def=Protein identification using a combination of [[wikipedia: | |def=Protein identification using a combination of [[wikipedia:high-performance liquid chromatography|high-performance liquid chromatography]] and [[mass spectrometry]] in which the proteins in a mixture are digested, usually by a specific enzyme, and the resulting peptides are separated by liquid chromatography and identified by [[tandem mass spectrometry]]. | ||
|rel=[[bottom-up proteomics]] | |rel=[[bottom-up proteomics]] | ||
|ref= | |ref=M. L. Fournier, J. M. Gilmore, S. A. Martin-Brown, M. P. Washburn. Chem. Rev. 107, 3654 (2007). (http://dx.doi.org/10.1021/cr068279a ) | ||
}} | }} | ||
Revision as of 13:27, 8 January 2014
| IUPAC RECOMMENDATIONS 2013 |
| Shotgun proteomics |
|---|
| Protein identification using a combination of high-performance liquid chromatography and mass spectrometry in which the proteins in a mixture are digested, usually by a specific enzyme, and the resulting peptides are separated by liquid chromatography and identified by tandem mass spectrometry. |
| Related Term(s): bottom-up proteomics |
| Reference(s):
M. L. Fournier, J. M. Gilmore, S. A. Martin-Brown, M. P. Washburn. Chem. Rev. 107, 3654 (2007). (http://dx.doi.org/10.1021/cr068279a ) |
| From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013. |
