Peptide mass fingerprinting: Difference between revisions
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{{Final | {{Final | ||
|acronym= | |acronym=([[PMF]]) | ||
|def=Method for protein analysis where an unknown protein is chemically or enzymatically [[wikipedia:Proteolysis|cleaved]] into peptide fragments whose masses are determined by [[mass spectrometry]]. The peptide masses are compared to peptide masses calculated for known proteins in a database and analyzed statistically to determine the best match. | |def='''peptide mass fingerprinting''' | ||
'''[[mass mapping]]''' | |||
Method for protein analysis where an unknown protein is chemically or enzymatically [[wikipedia:Proteolysis|cleaved]] into peptide fragments whose masses are determined by [[mass spectrometry]]. The peptide masses are compared to peptide masses calculated for known proteins in a database and analyzed statistically to determine the best match. | |||
|rel=[[accurate mass tag]] | |rel=[[accurate mass tag]] | ||
|ref=D. J. C. Pappin, P. Hojrup, A. J. Bleasby. Curr. Biol. 3, 327 (1993). (http://dx.doi.org/10.1016/0960-9822(93)90195-T ) | |ref=D. J. C. Pappin, P. Hojrup, A. J. Bleasby. Curr. Biol. 3, 327 (1993). (http://dx.doi.org/10.1016/0960-9822(93)90195-T ) | ||
}} | }} | ||
[[Category:Proteomics]] |
Latest revision as of 17:45, 4 April 2014
IUPAC RECOMMENDATIONS 2013 |
Peptide mass fingerprinting (PMF) |
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peptide mass fingerprinting
Method for protein analysis where an unknown protein is chemically or enzymatically cleaved into peptide fragments whose masses are determined by mass spectrometry. The peptide masses are compared to peptide masses calculated for known proteins in a database and analyzed statistically to determine the best match. |
Related Term(s): accurate mass tag |
Reference(s):
D. J. C. Pappin, P. Hojrup, A. J. Bleasby. Curr. Biol. 3, 327 (1993). (http://dx.doi.org/10.1016/0960-9822(93)90195-T ) |
From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013. |