Bottom-up proteomics: Difference between revisions

Revision as of 18:21, 29 October 2013

Bottom-up proteomics
IUPAC RECOMMENDATIONS 2013
Method of protein identification that uses proteolytic digestion before analysis by liquid chromatography and mass spectrometry. Proteins can be isolated by gel electrophoresis prior to digestion or, in shotgun proteomics, the protein mixture is digested and the resulting peptides are separated by liquid chromatography.
Related Term(s): top-down proteomics
Reference(s): T. Baer, J. C. Morrow, J. D. Shao, S. Olesik. J. Am. Chem. Soc. 110, 5633 (1988). (http://dx.doi.org/10.1021/ja00225a008 )
From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.
Index of Recommended Terms

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-{{jchrom|
+{{Final
-A method of protein identification that uses proteolytic digestion before liquid chromatography and [[mass spectrometry]] analysis. Proteins can be isolated by gel electrophoresis prior to digestion or, in [[shotgun proteomics]], the protein mixture is digested and the resulting peptides are separated by liquid chromatography
+|acronym=
-|
+|def=Method of protein identification that uses proteolytic digestion before analysis by liquid chromatography and [[mass spectrometry]]. Proteins can be isolated by gel electrophoresis prior to digestion or, in [[shotgun proteomics]], the protein mixture is digested and the resulting peptides are separated by liquid chromatography.
-#N.L. Kelleher, H.Y. Lin, G.A. Valaskovic, D.J. Aaserud, E.K. Fridriksson, F.W. McLafferty, J. Am. Chem. Soc. 121 (1999) 806.
+|rel=[[top-down proteomics]]
-#B.T. Chait, Science 314 (2006) 65.
+|ref=T. Baer, J. C. Morrow, J. D. Shao, S. Olesik. J. Am. Chem. Soc. 110, 5633 (1988). (http://dx.doi.org/10.1021/ja00225a008 )
 }}