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Comparative Analysis of Conventional Cell Lysis Techniques to Electrochemical Cell Lysis using the Liquid Microjunction Surface Sampling Probe

Cell lysis is a critical step in the analysis of cellular components, influencing the efficiency, reproducibility, and integrity of biomolecular extractions. Conventional lysis techniques, including mechanical and chemical methods, often present trade-offs between throughput, scalability, and sample integrity. Mechanical approaches such as bead milling and sonication can efficiently disrupt cells but suffer from issues such as back-mixing and biomolecule degradation. Chemical lysis technique...

— Rosa, Perentesis 2025-12-15 00:00:00

Measurable Feature Prediction for Estimating Chemical Space Coverage in LC-ESI-HRMS Non-targeted Analysis

The chemical space, encompassing both known and theoretically possible compounds, continues to expand thanks to anthropogenic activity and transformation processes. Understanding which regions of this vast space are experimentally measurable is critical for advancing non-targeted analysis (NTA) for exposomics and environmental monitoring. In practice, comprehensive NTA by LC–ESI–HRMS is constrained by method-specific conditions, such as retention and ionization, thus defining subspace reg...

— Saer, Samanipour 2025-12-14 00:00:00

Depth-Resolved Lithium Isotope Fractionation as a Diagnostic of Interphase Evolution and Degradation in Lithium Ion Batteries

Lithium isotopic fractionation is well-established in dynamic geochemical systems; however, its role in lithium-ion batteries (LIBs) remains uninvestigated. Herein, we report the first depth-resolved demonstration that isotopic separation occurs during Li-ion cell operation whose magnitude depends on the cycling history. Using depth-resolved glow discharge mass spectrometry, we monitored the 7Li/6Li ratio in LiNi0.333Mn0.333Co0.333O2 (NMC111)||graphite coin cell electrodes at defined life-cyc...

— Sebastian, Recknagel 2025-12-12 00:00:00

Gas chromatography-mass spectrometry (GC-MS) in the plant metabolomics toolbox. Part 2: Analysis of polar low molecular weight metabolites

Decades ago, the introduction of GC-MS marked a significant advancement in primary plant metabolites studies. Here, in Part 2 of the review, we will delve into critical aspects of the workflow, spanning the selection of an analytical platform, sample preparation, analytical acquisition, and data processing and interpretation. The exceptional separation capabilities of GC, characterized by remarkable chromatographic resolution, rendered it ideal for analysis of the complex plant metabolome, in...

— Andrej , Frolov 2025-12-12 00:00:00

On Trapping of Ions Using Low E/N Rotating Electric Fields: Towards All-Pressure Ion Confinement

Here, we introduce a novel approach that enables ion confinement at atmospheric pressure and extends it generally low E/N conditions. We show ion trapping at atmospheric pressure by suspending ions within dynamic rotating electric fields. More broadly, we report ion confinement under low E/N conditions with applied voltages rotating at kHz frequencies, where ions are thermalized and confined by being constantly passed over by a rotating wave that causes them to move angularly forward and roll...

— Yehia, Ibrahim 2025-12-12 00:00:00

The Potential of Machine Learning in Oligonucleotide Therapeutics Manufacturing

Approvals of synthetic oligonucleotide therapeutics are sharply rising and with it the challenges to achieve sustainable manufacturing strategies. These chain-like molecules are commonly assembled via solid phase oligonucleotide synthesis (SPOS), a method that allows for quick and efficient sequential additions of monomers up until the desired molecule length. A primary challenge in SPOS is the meticulous control of the multitude of variables at each additional step to avoid formation of comp...

— Emma, Green 2025-12-12 00:00:00

Global inventory of fluoropolymer production plants and their associated PFAS environmental contamination

Fluoropolymers are widely used across sectors, but their production is associated with emissions of per- and polyfluoroalkyl substances (PFASs), which are mobile, persistent, and toxic. In this work, we compiled a global inventory of fluoropolymer production plants (FPPs) and assembled PFAS concentration measurements for various media in their vicinity. We identified 52 currently operating FPPs across 11 countries and 41 cities, with 12 cities having peer-reviewed site-specific PFAS measureme...

— Martin, Scheringer 2025-12-11 00:00:00

Multiple Heart-Cut IEC-RPLC Platform for Online Desalting and Fractionation of Monoclonal Antibody Charge Variants

Minor deviations in biopharmaceutical manufacturing processes, such as those used for monoclonal antibodies (mAbs), can introduce structural modifications that alter protein efficacy and safety. Monitoring these changes is critical to ensure product consistency and efficacy. Ion-exchange chromatography (IEC) is widely employed to assess product quality by separating charge variants. However, the use of non-volatile salts in IEC makes direct identification and characterization of these variant...

— Andrea F., Gargano 2025-12-11 00:00:00

Prioritisation of persistent known and unknown chemicals using synthetic accessibility and tandem mass spectrometry

The vast and expanding chemical universe contains hundreds of thousands of substances, yet experimental data on their environmental persistence are available for only a small fraction (<3%). This gap limits our ability to identify persistent organic contaminants. Models have been developed to predict the persistence for already identified chemicals, but no models are able to prioritise unknown features detected in high-resolution mass spectrometry (HRMS). Here, we present two complementary ma...

— Jake, O'Brien 2025-12-11 00:00:00

Identification of Blood Biomarkers for Bipolar Disorder using SERS and Explainable Machine Learning

The differentiation of bipolar disorder (BD) from major depressive disorder (MDD) represents a persistent challenge in psychiatry, leading to high rates of misdiagnosis and adverse patient outcomes. Objective biomarkers are critically needed, but their discovery is hampered by limited understanding of the disease etiology. Robust unbiased, hypothesis-free, and untargeted discovery methods combined with machine learning could prove valuable in providing diagnostic utility. In this study, a nov...

— Oren A., Scherman 2025-12-11 00:00:00

Lithium isotope fractionation as an early indicator of degradation mechanisms in lithium-ion batteries

Aging in lithium-ion batteries (LIBs) degrades the performance and hinders sustainability, demanding advanced diagnostics for early failure prediction. We investigate lithium isotope fractionation (LIF) as an innovative probe of degradation in lithium cobalt oxide (LCO) coin cells aged over 0−700 cycles. High-precision multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS) quantified δ7Li variations across key cell stages: non-cycled (0 cycles), newly formed (5 cycles), s...

— Ulrich, Panne 2025-12-10 00:00:00

Fluorosequencing without terminal residue cleavage

A modification to fluorosequencing, an emerging method for large-scale parallel protein identification, is proposed. In this modified approach terminal residues are not cleaved by Edman degradation (a key step in fluorosequencing) or any of its variants or enzymatic alternatives, the process is non-destructive. There are three parts: 1) a charged homopeptide header is attached to each of the peptides being partially sequenced; 2) the glass slide that the headers are immobilized on is mounted ...

— G, Sampath 2025-12-10 00:00:00

Operando Microscopy of Photosynthetic Microbial Biohybrids using Fluorescent Chemical Probes

Photosynthetic biohybrids, often in the form of biophotoelectrochemical devices, aim to achieve solar-to-chemical conversion by pairing biotic and abiotic materials, leveraging the beneficial attributes of both. Numerous works have highlighted the importance of a well-tuned bio-electrode interface for high biophotoelectrode performance. However, the dynamics of these interfaces in photosynthetic biohybrids are poorly understood, necessitating the development of new tools to disentangle the va...

— Jenny Z., Zhang 2025-12-10 00:00:00

Between Overlook and Oversight: Misconceptions in Concentration for Battery Electrolyte

Accurate concentration of electrolytes is essential for ensuring the reliability of battery research and development. Recent studies in electrolytes often overlook or overshoot concentration, leading to widespread misuse and misapplication of concentration definitions. The aim of this article is to highlight the impact of these concentration miscalculations on battery research performance and to propose a clearer, more accurate framework for electrolyte formulation.

— Kazuhiko, Matsumoto 2025-12-10 00:00:00

Direct-inject suppressed ion chromatography-mass spectrometry method with online preconcentration for short- and ultra short-chain perfluoroalkyl carboxylic acids in fresh water

This work developed a novel analytical method to detect short- and ultra-short chain perfluoroalkyl carboxylic acids (PFCAs) in freshwater samples by direct injection using ion chromatography mass spectrometry (IC-MS). Ultra-short chain PFCAs, including trifluoroacetic acid (TFA), are often present in aqueous environments at higher concentrations than the longer chain PFCAs, however there are currently a limited number of methods that can analyze them. Detection limits ranged between 1.3 – ...

— Jessica, Clouthier 2025-12-09 00:00:00

Investigation of the robustness of Rayleigh optical activity for the assignment of absolute configurations of chiral molecules

Experimental measurements of Rayleigh optical activity (RayOA) for liquid phase chiral molecules have been recently reported for the first time, nearly 50 years after it was theoretically formulated. Inspired by this experimental data, we computationally model the RayOA of several chiral molecules to assess the usefulness of this newly reported experimental method for assigning their absolute configurations. We consider the influence of factors that can often preclude the routine assignment o...

— Laurence, Barron 2025-12-09 00:00:00

Pinpointing non-covalent lipid binding sites in membrane proteins via native top-down mass spectrometry

Pinpointing non-covalent lipid-binding sites remains a major challenge in structural biology. Lipids act as dynamic cofactors that regulate membrane protein folding, function, and drug interactions, yet their precise localization is often inaccessible. Here, we introduce a capillary electrophoresis–native top-down mass spectrometry (CE–nTDMS) platform that preserves and maps labile membrane protein–lipid interactions at residue-level resolution. Using Aquaporin Z, a tetrameric transmemb...

— Brian , Fuglestad 2025-12-08 00:00:00

A new beginning in Molecular Imprinting: Revealing Key Experimental Variables Shaping the Mechanochemical Synthesis of Molecularly Imprinted Polymers

Molecularly imprinted polymers (MIPs) are tailor-made functional polymers increasingly acknowledged for their strong performance across scientific fields. However, conventional MIP synthesis often relies on complex chemical processes that hinder sustainable scale-up and industrial adoption. To address this challenge, modern production strategies should integrate advances in polymer science and industrial processing technologies to achieve greener and more scalable manufacturing. Solventless a...

— Lucian, Barbu-Tudoran 2025-12-08 00:00:00

Lyophilised colourimetric LAMP for visual readout with dual colour indicators

Loop-mediated isothermal amplification (LAMP) is increasingly recognised as a practical alternative to PCR for pathogen detection due to its shorter turnaround time, constant operating temperature, and compatibility with a wide range of detection methods. Colourimetric LAMP has gained popularity due to its potential for instrument-free readout, making it attractive for molecular diagnostics in low-resource settings. Despite these advantages, the widespread adoption of colourimetric LAMP at th...

— Jesus, Rodriguez-Manzano 2025-12-08 00:00:00

From Biological Inspiration to Molecular Engineering: Carboxylate-Enhanced NDI Fluorophores for Mercury Sensing

Drawing inspiration from biomimetic design, increasingly recognized in developing organic molecules for sensing applications, this study tackles the challenge of selectively detecting mercury ions. Traditional detection methods often rely on sulfur-containing functional groups, such as thiol and disulfide linkages, which, despite their effectiveness, can compromise specificity due to cross-reactivity with other metal ions. This research explored the potential of incorporating oxygen and nitro...

— Praveen C, Ramamurthy 2025-12-08 00:00:00

SPOT-Based Ultrasensitive Visual Detection of Microbial Chitinolytic Activity Using a Nanofibrous N-Acetylchitosan Hydrogel

The objective of this work is to enable the ultrasensitive detection of microbial chitinolytic activity, addressing limitations of conventional halo assays which lack the sensitivity required to discover weakly active strains. To achieve this, we developed a visual sensing platform based on surface-pitting observation technology (SPOT) using a nanofibrous N-acetylchitosan hydrogel. When microorganisms are cultured on the gel surface, the hydrogel surrounding the cells is locally hydrolyzed by...

— Yasunori, Kurosawa 2025-12-08 00:00:00

SYNTHESIS AND CHARACTERIZATION OF HETEROCYCLIC N, N’- BIDENTATE LIGANDS AND THEIR TRANSITION METAL (II) COMPLEXES

This paper seeks to report the synthesis and characterization of N, N’-bidentate ligands together with their copper and zinc complexes. The reaction of equimolar quantities of the selected hydrazinyl pyridazines with a diketone resulted in the formation of the expected pyrazolylpyridazines. The transition metal (II) complexes were obtained by the reaction of the metal chlorides with the synthesized ligands in the ratio 1:2. The synthesized ligands and the complexes were characterized using ...

— ERIC CHOMBA, NJAGI 2025-12-07 00:00:00

Functionalization of saturated and unsaturated hydrocarbons with electrophilic anions [B12X11]– (X=Br, I) in the gas phase and at interfaces

Electrophilic dodecaborate fragment ions (X = halogen, CN) are among the most reactive anions and are capable of converting inert reagents into charged species through covalent bonding. In this study, we investigate the reactivity of [B12X11]– (X = Br, I) with a series of hydrocarbons in two environments: 1) In the gas phase, using ion–molecule reactions and collision-induced dissociation (CID), and 2) at interfaces, using fragment ion deposition onto surface layers of reagents. In the ga...

— Jonas, Warneke 2025-12-07 00:00:00

Multiplexed Enzymatic Biosensors with On-Chip pH Control for Lactate and pH Monitoring in Calf Saliva Operating Under an IoT Enabled Electronics Interface

Non-invasive biomarker assessment in saliva offers a promising route for early detection of bovine respiratory disease (BRD) and scour in calves. However, accurate electrochemical sensing in this complex matrix remains challenging due to variable pH, viscosity, and interfering species. Here, we report an integrated dual-function interdigitated microelectrode (IDE) platform capable of simultaneous detection of lactate and pH, incorporating a novel in-situ electrochemical pH control strategy th...

— Alan, O’Riordan 2025-12-07 00:00:00

From Spectra to Local Networks: Evaluating MS² Similarity Metrics

MS² spectral similarity is fundamental to interpreting LC-HRMS-NTA data. Beyond the commonly used cosine similarity, a wide range of alternative metrics, including distance-, probability-, and machine-learning–based approaches, provide different perspectives on spectral matching. In this study, we extracted 8,290 subdatasets from publicly available LC-HRMS/MS libraries (MassBank, MoNa, GNPS, and NIST), each containing spectra sharing a precursor m/z within a 5 mDa tolerance. We evaluated 2...

— Saer, Samanipour 2025-12-04 00:00:00

An integrative approach towards bio-inspired sequestration of rare earth elements

Despite their important role in the development of renewable energy, the process of obtaining lanthanides (Ln) through conventional mining or through recovery processes is problematic due to their negative impact on the environment. Research into new recovery processes therefore will be crucial to enable cost-effective and environmentally friendly enrichment and separation of Ln, particularly from waste streams or mine water. Many proteins contain specific peptide sequences as metal-binding s...

— Michael, Kumke 2025-12-03 00:00:00

Background-Free Nanopore Decoding of microRNA Expression Patterns Using Circular Diagnostic DNA

This paper describes a background-free nanopore decoding method to identify microRNA (miRNA) expression patterns that are both upregulated and downregulated. This method uses a circular DNA construct on MinION, a commercially available nanopore device. This system uses a pair of complementary diagnostic DNAs (dgDNAs) that form a closed structure too large to pass through the nanopore. This effectively prevents translocation and eliminates background signals. When the target miRNAs bind to dgD...

— Kazuhito, Satomura 2025-12-03 00:00:00

Uncovering Monitoring Gaps and Novel Persistent and Mobile Substances (PMs) in Groundwater Using Lyophilisation Enrichment and SFC-HRMS Smart Screening

Persistent and mobile chemicals (PMs) threaten groundwater quality and drinking-water safety, yet many remain undetected because analytical methods insufficiently address to highly polar and ionic substances, while regulatory frameworks lack monitoring requirements for these compound classes. Here, we developed a supercritical-fluid chromatography–high-resolution mass spectrometry (SFC-HRMS) based smart-screen approach that integrates lyophilisation enrichment, tiered suspect-screening stra...

— Thorsten , Reemtsma 2025-12-01 00:00:00

Soil Carbon Quantification by Shifted-Excitation Raman Difference Spectroscopy with Common-Mode Rejection

Quantifying soil organic carbon (SOC) has a vital role to play in advancing soil health, optimizing agricultural productivity, and helping mitigate climate change. Expanding the scope of traditional soil analysis necessary to realize this promise presents a significant challenge of scale. Spectroscopic methods, such as Raman scattering, offer higher throughput but encounter complications owing to sample heterogeneity, matrix interactions, and interfering fluorescence. In this study, we integr...

— Edward, Grant 2025-12-01 00:00:00

Differentiation-Based Coats–Redfern Framework for Conversion-Resolved Kinetics: Application to Catalytic and Non-Catalytic Polyethylene Thermogravimetry

Classical Coats-Redfern (CR) framework linearizes the integral rate equation and returns a single set of kinetic parameters, activation energy (Ea), pre-exponential factor (A), and rate profile (dα/dt), averaged over a fixed conversion (α) interval, which obscures conversion resolved features and mixing overlapping mechanistic steps. In this study, we introduce differentiation-based Coats-Redfern (d-CR) framework that replaces linearization step of the classical CR framework for the depen...

— Young Soo, Kang 2025-12-01 00:00:00

Current-controlled Zinc Electrodeposition Morphology in Ionic Liquid Electrolytes using Microelectrode Arrays

Understanding and controlling the microstructure of zinc (Zn) metal electrodeposits is critical for advancing the next generation of rechargeable Zn batteries. In this study, we develop microelectrode arrays to systematically investigate the relationship between current density, morphology, and Coulombic efficiency (CE) during Zn electrodeposition from an ionic liquid electrolyte. By independently controlling the current density or voltage on each microelectrode, we identify three key deposit...

— Yiyang, Li 2025-11-30 00:00:00

Propionic acid outperforms formic and acetic acid in MS sensitivity for high-flow reversed-phase LC-MS bottom-up proteomics

Formic acid has long been the default acidic additive in reversed-phase LC-MS-based bottom-up proteomics, offering a practical balance between chromatographic performance and electrospray ionization (ESI) efficiency. Here, we evaluate propionic acid as an alternative mobile phase acidifier, a candidate that has been largely overlooked in efforts to improve ESI efficiency without compromising chromatography. By reducing both the ionic strength and surface tension of the mobile phase, propionic...

— Jan, Rasl 2025-11-28 00:00:00

Upcycling Rare Earth Elements from Leached Phosphor Fractions (YOX, LAP) Utilizing α-Aminobisphosphonate

A process involving three phases (leaching, purification, and precipitation) for upcycling rare earth elements (REEs) from spent fluorescent lamp phosphor material was developed. First, the leaching of different phosphor fractions using methanesulfonic acid (MSA) as a lixiviant was studied. The optimized three-step leaching sequence involved: (1) leaching the halophosphonate fraction (HALO) with pure MSA (L/S=20 L/kg, T=22 °C, t= 1 h), (2) leaching the yttrium-oxide (YOX) fraction with 5% MS...

— Ari, Väisänen 2025-11-28 00:00:00

In situ polymer nanoparticle densitometry via real-time 3D single-particle tracking

This work introduces a single-particle method for measuring the density and mass of freely diffusing polymer nanoparticles during ring-opening metathesis polymerization of norbornene. Using 3D Single-Molecule Active Real-time Tracking (3D-SMART), growing polymer nanoparticles are tracked in situ, enabling real-time, simultaneous measurement of both the particle size and, for the first time, particle density. Particle density is extracted for the descent velocity of growing nanoparticles, givi...

— Donggeng, Yu 2025-11-28 00:00:00

Development of MALDI-TOF-MS method for identification of free amino acids in human tears

The anterior cornea is a primary optical surface of the eye and is bathed in tears that play blood-like roles to this avascular tissue. The functions of tears suggests that tear composition can be used to track and monitor biomarkers of the physiological state of the cornea. While there are various means to collecting tears for analysis, there is a need to develop methods of analysis well suited to particular tear chemical content. The purpose of this study is to demonstrate an optimized meth...

— Scott , Shippy 2025-11-27 00:00:00

Direct Quantum Yield Measurement of a Ligand-to-Metal Charge Transfer Photocatalyst via Cyclic Voltammetry

Measurements of molecular quantum yields are crucial for understanding and directing photocatalytic activation of inert substrates. However, present methods to study photocatalysts are limited by high costs, disparate measurement and photocatalytic reaction timescales, or poor luminescent behavior. Electroanalytical methods such as cyclic voltammetry (CV) are a promising alternative to address these limitations. Here, CV was used to directly measure the molecular quantum yield of a model iron...

— Brandon, Grasty 2025-11-26 00:00:00

Cumulative Spectroscopic Detection for Taylor Dispersion Analysis of Nanoparticles

Taylor Dispersion Analysis (TDA) provides a robust approach for determining the hydrodynamic radius of molecular objects and nanoparticles (NPs), measuring diffusion of the analyte in laminar flow through a long capillary. TDA measurements are usually performed on capillary electrophoresis equipment with in-line absorbance detection. Here, we introduce a simple, yet versatile cumulative spectroscopic detection (CSD) technique for TDA, that precisely samples the radially averaged analyte conce...

— Niko, Hildebrandt 2025-11-26 00:00:00

Soft ionization mass spectrometry techniques: the motion laws of gaseous ions in weak electric fields

Ionization mass spectrometry technology has been studied for a relatively long time, and its theoretical development is relatively slow compared to chromatography and other fields. This article is based on the author's theory, establishes a corresponding model, introduces the equation for calculating the resistance coefficient of particles in the air, and explores the motion law of gas-phase ions in weak electric fields. Through the derivation of equations, mathematical equations were used to...

— Jiehong, Luo 2025-11-26 00:00:00

LipiSonics: An Enhanced High-Throughput Lipidomics Workflow Using Acoustic Ejection Mass Spectrometry

Lipidomic studies demand analytical platforms capable of capturing the structural diversity and complexity of lipid species with accuracy, precision, and speed. The Echo® MS+ system, an advanced acoustic ejection (AE) system coupled with a mass spectrometer (MS), offers a genuinely high-throughput, contactless workflow for comprehensive lipid profiling. In this study, we developed an automated high-throughput workflow based on a single-phase lipid extraction using 1-octanol, and methanol wi...

— Hyungwon, Choi 2025-11-26 00:00:00

Intermolecular Nuclear Spin Hyperpolarization Transfer via Cross-Relaxation Triggers RASER of Solute Molecules

Radiofrequency amplification by stimulated emission of radiation (RASER) is a phenomenon that is observed in systems of nuclear spins with strong inverse polarization beyond the thermal equilibrium. RASER is of fundamental interest and also possesses several features of possible practical importance, such as very narrow NMR lines and background-free detection. However, so far, the effect was limited to molecules directly polarized by corresponding hyperpolarization techniques. In this work, w...

— Igor, Koptyug 2025-11-25 00:00:00

Profiling glycoproteins enriched by multi-nanoparticle protein corona

Biofluids, such as plasma and cerebrospinal fluid (CSF), present significant challenges for proteome and glycoproteome analysis due to their complex protein composition and the wide dynamic range of protein abundances. Nanoparticle (NP)-based enrichment methods that rely on the formation of protein coronas to compress dynamic range have emerged as promising approaches to improve the proteomic sampling depth in biofluids, yet implications of these methods for glycoproteome characterization rem...

— Nicholas M., Riley 2025-11-25 00:00:00

Operando SAXS Investigation of Hydrated Membrane Nanostructure in Polymer Electrolyte Fuel Cells

Understanding the hydration state of polymer electrolyte membranes (PEMs) in operating fuel cells is essential for enhancing performance and ensuring long-term durability. Here, we present an operando small-angle X-ray scattering (SAXS) approach that enables direct observation of PEM hydration within membrane electrode assemblies. A dedicated operando SAXS cell was developed to suppress scattering from catalyst layers and isolate membrane-specific signals. Operando measurements revealed that ...

— Hiroshi, Nozaki 2025-11-24 00:00:00

Microscale Native Size Exclusion Chromatography High Resolution Mass Spectrometry for the Determination of the Chelator-To-Antibody Ratio of Immunoconjugates

Positron emission tomography (PET) using radiolabeled antibodies (radioimmunoconjugates) is a powerful imaging technique to track the distribution of therapeutic antibodies. Accurate determination of the average number of chelators per antibody (CAR) is essential for (radio)immunoconjugate characterization. Radiometric titration, although widely used, is labor-intensive and requires approximately half a day per sample. To overcome this analytical bottleneck, we developed a microscale size exc...

— Andrea F.G., Gargano 2025-11-24 00:00:00

Hunting of Endogenous and Exogenous Radical Species into DNA Cleavage Catalysed by a Mn2+-dependent Deoxyribozyme

DNAzymes, catalytically active DNA molecules, do perform DNA cleavage through metal ion co-factors (dia- and para-magnetic cofactors). The radical path through which the cleavage occurs is characterized by several radical species. Identify those species, as well as the fine tuning of the cofactors’ activities is a key role into the realm of such in vitro selected DNA-based enzymes. In this work, different radical species were identified, both for the cleavage reaction and for the stabilizat...

— Didier, Gasparutto 2025-11-22 00:00:00

Bridging the gap between positive and null outcomes in controversial microdroplet oxidation chemistry experiments

Recent observations of unexpected oxidation reactions occurring in the presence of water microdroplets that otherwise do not occur in bulk water challenge our understanding of interfacial water chemistry. If such processes are occurring generally in water-based droplets suspended in air, there are remarkable implications for atmospheric chemistry, sustainability, and airborne disease transmission, among others. These observations, however, remain controversial. Some investigators have been un...

— Ryan, Davis 2025-11-21 00:00:00

Skin swab sampling for quantitative mass spectrometry-based diagnostics

Skin swab sampling offers an accessible and non-invasive sample collection approach with applications in biomarker discovery and diagnostic testing. This study investigates the robustness of skin swab sampling and the feasibility of quantifying skin surface components using Liquid Chromatography-Mass Spectrometry. A semi-quantitative analysis and tandem mass spectrometry with Multiple Reaction Monitoring (MRM) for targeted quantification were used. Skin swabs were collected, stored and extrac...

— Michael, Morris 2025-11-21 00:00:00

Native multi-dimensional protein complex identification and topology characterization (Native MudPIT)

Here, we demonstrate pilot development of native multi-dimensional protein complex identification and topology characterization (Native MudPIT) in complex biological samples via coupling native size exclusion chromatography (SEC), online native capillary electrophoresis-mass spectrometry (nCE-MS), surface-induced dissociation (SID), and multi-level proteomics. With semi-manual data interpretation because optimized algorithms do not yet exist for this approach, the Native MudPIT approach enabl...

— Fei, Fang 2025-11-21 00:00:00

Novel Stability-Indicating Assay for Palbociclib: A Breakthrough in Anti-Breast Cancer Therapy

Palbociclib, a cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitor, plays a crucial role in the treatment of hormone receptor-positive, HER2-negative metastatic breast cancer. Ensuring the accurate quantification and stability of Palbociclib in pharmaceutical formulations is critical for maintaining therapeutic efficacy and patient safety. Existing analytical methods often lack the precision, robustness, and cost-effectiveness required for routine quality control. In this study, a novel revers...

— Kirankumar, Nalla 2025-11-21 00:00:00

Evaluating C2-Neighbouring and C3-, C4-, C6-Remote Participation in Peracetylated Glycosyl Cations

Neighbouring group participation and remote participation are fundamental concepts in carbohydrate chemistry and are commonly applied to achieve stereocontrol in glycosylation reactions. The corresponding intermediates can be glycosyl cations, which are challenging to characterise due to their short-lived nature. Using gas-phase infrared spectroscopy supported by density functional theory calculations, we directly assess and rank the participation modes of acetyl protecting groups in peracety...

— Kevin, Pagel 2025-11-20 00:00:00

Single-Particle Electrochemistry for Active Matter: Advancing the Detection and Analysis of Artificial Micromotors, Bacteria and Enzymes

Single-particle electrochemistry (SPE) has emerged as a transformative analytical technique enabling real-time, high-resolution detection of micro-/nanoscale entities, including synthetic micro-/nanomotors (MNMs), bacteria and enzymes. By capturing transient electrochemical signals from stochastic particle-electrode collisions, SPE provides insights into reaction kinetics, charge transfer mechanisms and catalytic behavior at the single-entity level. This review presents a comprehensive overvi...

— Pramit Kumar , Chowdhury 2025-11-20 00:00:00